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Eğitim, biyogüvenlik kurallarını ve COVID-19 testlerinin güvenli şekilde nasıl yapılacağını anlatmaktadır.","Video, biyogüvenlik kavramının tanımıyla başlayıp, dört biyogüvenlik düzeyini detaylı olarak açıklamaktadır. Ardından tehlike ve risk kavramları arasındaki ilişki örneklerle anlatılmakta, SARS-CoV-2'nin bulaşma yolları ve özellikleri açıklanmaktadır. Daha sonra hangi testlerin hangi biyogüvenlik düzeyinde çalışılması gerektiği, laboratuvar tasarımı, ekipman kullanımı, kişisel koruyucu ekipmanlar ve analiz öncesi, analiz ve analiz sonrası süreçlerde alınması gereken önlemler detaylı şekilde ele alınmaktadır."]},"endTime":606,"title":"SARS-CoV-2 Tanı Laboratuvarlarında Biyogüvenlik Eğitimi","beginTime":0}],"fullResult":[{"index":0,"title":"Biyogüvenlik Nedir ve Düzeyleri","list":{"type":"unordered","items":["Biyogüvenlik, potansiyel patojenik tehlike içeren materyal, enfeksiyöz mikroorganizmalar veya onların genetik/toksik komponentleri ile yapılan çalışmaların güvenli şekilde yapılmasını sağlamak amacıyla laboratuvar altyapısı, tasarım, ekipman, teknik ve uygulamaların kombinasyonudur.","Biyolojik risklerin kontrolüne yönelik uygulamalar, laboratuvarın fiziksel koşulları ve güvenlik donanımlarına göre dört biyogüvenlik düzeyi tanımlanmıştır.","Biyogüvenlik düzeyi bir, sağlıklı ilişkilerde hastalık oluşturmayan mikroorganizmalarla çalışmak için uygun seviyedir; düzeyi iki, çalışana ve çevreye zarar verme potansiyeline sahip etkenlerle çalışmak için uygun seviyedir; düzeyi üç, çalışanlar için ciddi risk oluşturan ve topluma yayılma potansiyeline sahip etkenlerle çalışmak için uygun seviyedir; düzeyi dört, aerosol yoluyla bulaşan ve ölümcül enfeksiyonlara neden olabilen ajanların çalışıldığı seviyedir."]},"beginTime":1,"endTime":120,"href":"/video/preview/822490914502001911?parent-reqid=1769008146483049-1600396887520451139-balancer-l7leveler-kubr-yp-vla-33-BAL&text=CBS+LABORATUVAR&t=1&ask_summarization=1"},{"index":1,"title":"Tehlike ve Risk Kavramları","list":{"type":"unordered","items":["Tehlike kavramı çalışanlara, diğer kişilere, laboratuvar ortamına ve çevreye zarar verebilecek her şeyi içerirken, risk kavramı bir tehlikenin olumsuz bir sonuca neden olma olasılığını ifade eder.","Risk değerlendirme yaklaşımı, riskin gerçekleşme olasılığı ve risk gerçekleştiği takdirde ortaya çıkacak sonucun şiddeti temelinde görece ve öznel olan laboratuvardaki risklerin belirlenmesine yönelik sistematik bir yaklaşımdır.","COVID-19 hastalığı damlacık yoluyla bulaşmaktadır ve hasta bireylerin öksürme, hapşırma yoluyla ortaya saçtıkları damlacıklara diğer kişilerin elleriyle temas etmesi ve sonrasında ellerini ağız, burun veya göz mukozasına götürmesi ile de bulaşmaktadır."]},"beginTime":120,"endTime":190,"href":"/video/preview/822490914502001911?parent-reqid=1769008146483049-1600396887520451139-balancer-l7leveler-kubr-yp-vla-33-BAL&text=CBS+LABORATUVAR&t=120&ask_summarization=1"},{"index":2,"title":"COVID-19'un Bulaştırıcılık Özellikleri","list":{"type":"unordered","items":["İlk olguların epidemiyolojik özellikleri incelendiğinde inkübasyon süresinin ortalama 5-6 gün olduğu, bazı vakalarda 14 güne kadar uzayabileceği gözlenmiştir.","COVID-19'un bulaştırıcılık süresi kesin olarak bilinmemektedir, semptomatik dönemden 1-2 gün önce başlayıp semptomların kaybolmasıyla sona erdiği düşünülmektedir.","Genel olarak koronavirüsler dış ortama çok dayanıklı olmayan virüslerdir, cansız yüzeylerde birkaç saat içerisinde aktivitemi kaybettiği kabul edilmektedir."]},"beginTime":190,"endTime":226,"href":"/video/preview/822490914502001911?parent-reqid=1769008146483049-1600396887520451139-balancer-l7leveler-kubr-yp-vla-33-BAL&text=CBS+LABORATUVAR&t=190&ask_summarization=1"},{"index":3,"title":"Biyogüvenlik Düzeylerine Göre Testler","list":{"type":"unordered","items":["Tüm numunelerin ilk işlemesi onaylanmış, tercihen sınıf 2.1 biyolojik güvenlik kabininde yapılmalıdır.","Sekanslama, nükleer krista amplikasyon testi gibi bulaştırıcı olmayan tanısal çalışmalar biyogüvenlik düzeyi 2 laboratuvarlarda yapılmalıdır.","Virüs kültürü, izolasyonu veya nötralizasyon deneyleri gibi bulaştırıcı testler ise içeri doğru hava akışı bulunan biyogüvenlik düzeyi 3 laboratuvarlarda yapılmalıdır."]},"beginTime":226,"endTime":253,"href":"/video/preview/822490914502001911?parent-reqid=1769008146483049-1600396887520451139-balancer-l7leveler-kubr-yp-vla-33-BAL&text=CBS+LABORATUVAR&t=226&ask_summarization=1"},{"index":4,"title":"Laboratuvar Tasarımı ve Organizasyon","list":{"type":"unordered","items":["Laboratuvarlara yetkili olmayan hiçbir kimsenin girmemesi gereklidir ve laboratuvar girişleri bunu sağlamak üzere yapılandırılmalıdır.","Moleküler mikrobiyoloji laboratuvarları temiz alan ve kirli alanı olarak ayrılmalıdır, numune kabul birimi haricinde bu laboratuvarlar birbirinden ayrı ve mümkünse birbirine içerden geçiş olmayan en az üç farklı oda şeklinde tasarlanmalıdır.","Her odada kullanılan cihaz, ekipman ve sarf malzeme o odaya özgü olmalı ve odada kalmalıdır."]},"beginTime":253,"endTime":282,"href":"/video/preview/822490914502001911?parent-reqid=1769008146483049-1600396887520451139-balancer-l7leveler-kubr-yp-vla-33-BAL&text=CBS+LABORATUVAR&t=253&ask_summarization=1"},{"index":5,"title":"Laboratuvar Odaları ve Kontaminasyon Önleme","list":{"type":"unordered","items":["Xtraksiyon odası, numunelerin ilk açıldığı ve viral RNA'nın serbest kalması için hücrelerin parçalanma işleminin gerçekleştirildiği odadır.","Mix hazırlama odası, PCR reaktifleri ve ali kodlarını saklandığı ve PCR master mix'in hazırlandığı odadır.","Antifikasyon odası, real time PCR cihazının yer aldığı ve aplikasyon işlerinin gerçekleştirildiği odadır.","Kontaminasyonun önlenmesi için temiz odaya asla PCR ürünü dönüşü olmamalıdır ve kirli odaya ait cihaz, ekipman, sarf malzeme, flash bellek, dizüstü bilgisayar gibi malzemeler temiz odaya kesinlikle taşınmamalıdır."]},"beginTime":282,"endTime":329,"href":"/video/preview/822490914502001911?parent-reqid=1769008146483049-1600396887520451139-balancer-l7leveler-kubr-yp-vla-33-BAL&text=CBS+LABORATUVAR&t=282&ask_summarization=1"},{"index":6,"title":"Kişisel Koruyucu Ekipmanlar","list":{"type":"unordered","items":["Laboratuvar alanlarında çalışan tüm personel biyogüvenlik düzeyi şartları için gerekli olan gözlük, eldiven, maske gibi kişisel koruyucu ekipmanları kullanmalıdır.","Laboratuvar teknik alanına girmeden önce personelin kişisel koruyucu ekipmanlarının bulunduğu ve giyindikleri bir personel hazırlık odası bulunmalıdır.","Nükleer amplikasyon çalışmalarına başlamadan önce kişisel koruyucu ekipmanlar uygun sırayla giyilmeli ve çalışma sonrasında laboratuvardan çıkmadan uygun sıra ile çıkarılmalıdır.","COVID-19 testlerinin çalışıldığı laboratuvarda çalışan personel için bulunması gereken asgari kişisel koruyucu ekipman: uzun kollu, bileği dar gövde kısmı dize kadar uzun forma veya laboratuvar önlüğü, N95 veya N99 respiratör maske, pudrasız eldiven, sıvı geçirmez tulum veya tek kullanımlık önlük, bone, galoş, gözlük veya siperlik."]},"beginTime":329,"endTime":386,"href":"/video/preview/822490914502001911?parent-reqid=1769008146483049-1600396887520451139-balancer-l7leveler-kubr-yp-vla-33-BAL&text=CBS+LABORATUVAR&t=329&ask_summarization=1"},{"index":7,"title":"Laboratuvar İçindeki Güvenlik Önlemleri","list":{"type":"unordered","items":["Laboratuvardan ayrılmadan önce tercihen ılık su ve sabunla eller iyice yıkanmalıdır.","Yeme-içme, sakız çiğneme, sigara içme ve kozmetik uygulama gibi faaliyetler laboratuvar içinde gerçekleştirilmemelidir.","Eldiveni yırtabilen, kolayca kontamine olabilen ve enfeksiyon için odak görevi görebilecek takılar çıkarılmalı, tırnaklar kısa tutulmalıdır.","Tüm biyolojik ajanların ve kimyasal madde ve radyoaktif materyalin uygun şekilde etiketlenmesi sağlanmalıdır.","Mobil elektronik cihazlar, kolayca kontamine olmayacakları veya enfeksiyon odağı gibi hareket edemeyecekleri yerlerde tutulmalıdır.","Bu tür cihazların biyolojik ajanlara çok yakın olmasının kaçınılmaz olduğu durumlarda ise laboratuvardan çıkarılmadan önce fiziksel bir bariyerle korunduğundan veya dekontamine edildiğinden emin olunmalıdır.","Eldivenli ellerin yüz, göz, kulak ve burun gibi organlarla temasından kaçınılmalıdır."]},"beginTime":386,"endTime":444,"href":"/video/preview/822490914502001911?parent-reqid=1769008146483049-1600396887520451139-balancer-l7leveler-kubr-yp-vla-33-BAL&text=CBS+LABORATUVAR&t=386&ask_summarization=1"},{"index":8,"title":"Analiz Öncesi Süreç","list":{"type":"unordered","items":["Analiz öncesinde hastalığın örnek alınması sürecinde N95 FFP2 maske, yüz siperliği ve diğer koruyucu ekipmanlar usulüne uygun kullanılmalıdır.","Örnekler uygun kaplara alınmalı ve standart etiketlemeler yapılmalıdır.","COVID-19 şüpheli örnekler posterde tariflendiği şekilde üçlü paketleme sistemi ile taşınmalıdır ve kesinlikle pnömatik tüp taşıma sistemi ile nakledilmemelidir.","Numune kabul alanlarında temas mesafesi korunarak numune kabulünün yapılması sağlanmalıdır.","Laboratuvara gönderilmiş tüm örnek kapları uygun askeri kişisel koruyucu ekipman kullanılarak kabul edilmelidir.","Örneklerin alınması ve taşınmasında olduğu gibi laboratuvar içindeki hareketi ve çalışılması da laboratuvara gelen diğer örneklerden ayrı bir organizasyon ile gerçekleştirilmelidir.","Tüm örneklerin ilk işlemleri biyogüvenlik düzeyi 2 şartlarının sağlandığı alanda yapılmalıdır.","Sıçrama, damlacık veya aerosol oluşumuna neden olabilecek durumlar da dahil olmak üzere, potansiyeli olarak bulaşıcı malzemenin tüm manipülasyonları biyogüvenlik kabinlerinde çalışılmalıdır."]},"beginTime":444,"endTime":512,"href":"/video/preview/822490914502001911?parent-reqid=1769008146483049-1600396887520451139-balancer-l7leveler-kubr-yp-vla-33-BAL&text=CBS+LABORATUVAR&t=444&ask_summarization=1"},{"index":9,"title":"Xtraksiyon İşlemi ve Analiz Sonrası Süreç","list":{"type":"unordered","items":["SARS-CoV-2 ekstruksiyon işlemi süreçlerinde pipetleme, liz tamponu ekleme, ali kot hazırlama, numuneleri seyreltme, vorteksleme gibi tüm teknik prosedürler aerosol ve sıçrama oluşumunu en aza indirecek şekilde sınıf 2.1 güvenlik kabini içerisinde yapılmalıdır.","Numunelerin santrifüjlenmesi gerektiğinde kapaklı santrifüj motorları ve numune kapları kullanılmalı ve bu işlemler de biyogüvenlik kabini içinde gerçekleştirilmelidir.","Biyogüvenlik kabini içinde işlemi biten numune kapları, yüzeyleri uygun dezenfektan ile dekontamine edildikten sonra biyogüvenlik kabininden çıkarılmalıdır.","Xtraksiyon işlemi uygulandıktan sonraki basamaklarda numunelerin bulaştırıcı özelliğinin olmadığı kabul edilse de kişisel koruyucu ekipman kullanılmalıdır.","Analiz sonrası süreçte kirli alana girmiş olan barkod etiketleri, laboratuvar defteri gibi enfeksiyon kaynağı olabilecek malzemeler ofis alanına nakledilmemelidir.","Ortaya çıkan tüm atıklar tehlikeli atık olarak kabul edilmeli ve buna oyularak bertaraf edilmelidir."]},"beginTime":512,"endTime":573,"href":"/video/preview/822490914502001911?parent-reqid=1769008146483049-1600396887520451139-balancer-l7leveler-kubr-yp-vla-33-BAL&text=CBS+LABORATUVAR&t=512&ask_summarization=1"},{"index":10,"title":"Dinlenme Alanları ve Kişisel Güvenlik","list":{"type":"unordered","items":["Laboratuvar çalışanları hasta örnekleriyle temas halinde oldukları için COVID-19 enfeksiyonu riski altındadırlar.","COVID-19'un insandan insana bulaşan bir enfeksiyon hastalığı olduğu unutulmamalıdır.","Bu nedenle özellikle dinlenme alanlarından maske, mesafe ve el hijyeni ile ilgili önlemler gevşetilmemelidir.","Dinlenme alanlarında kalabalık oluşturulmamalı, yeme-içme aktiviteleri bu işler için belirlenen alanlarda sosyal mesafeyi koruyarak 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